Objective: The use of technological devices emitting radio frequency fields(RFF) is increasing day by day. Nowadays, low-frequency RFF has begun to be used in cancer treatment as a non-invasive method. However, the time-dependent effects of high-frequency RFF on healthy and cancer cells are not yet clear.
This study aims to examine the time-dependent effects of 3200MHz RFF on human breast cancer (MCF-7) and healthy mouse fibroblast (L929) cell lines.Methods: MCF-7 and L929 cells were cultured with DMEM medium. RFF exposures were applied at 29.55dBm power levels (with Efield level 14V/m). MCF-7 and L929 cell lines were exposed to 3200 MHz RFF for 4 hours.
Cell viability levels were determined immediately after exposure and following a 2-hour post-exposure incubation period.Results: Immediately after 4 hours of 3200 MHz RFF exposure, cell viability decreased significantly by 12% in MCF-7 cells, with a further 15% decrease observed after a 2-hour post-exposure period.
In contrast, the L929 cell line exhibited a significant increase in viability, with levels rising by 27% immediately after exposure and by 16% following the 2-hour post-exposure period(p
